Dichlorinated and Brominated Rugulovasines, Ergot Alkaloids Produced by Talaromyces wortmannii.

نویسندگان

  • Lívia Soman de Medeiros
  • José Vinícius da Silva
  • Lucas Magalhães Abreu
  • Ludwig Heinrich Pfenning
  • Carolina Lúcia Silva
  • Sérgio Secherrer Thomasi
  • Tiago Venâncio
  • Karl-Heinz van Pée
  • Kristian Fog Nielsen
  • Edson Rodrigues-Filho
چکیده

UHPLC-DAD-HRMS based dereplication guided the detection of new halogenated alkaloids co-produced by Talaromyces wortmannii. From the fungal growth in large scale, the epimers 2,8-dichlororugulovasines A and B were purified and further identified by means of a HPLC-SPE/NMR hyphenated system. Brominated rugulovasines were also detected when the microbial incubation medium was supplemented with bromine sources. Studies from 1D/2D NMR and HRMS spectroscopy data allowed the structural elucidation of the dichlorinated compounds, while tandem MS/HRMS data analysis supported the rationalization of brominated congeners. Preliminary genetic studies revealed evidence that FADH₂ dependent halogenase can be involved in the biosynthesis of the produced halocompounds.

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عنوان ژورنال:
  • Molecules

دوره 20 9  شماره 

صفحات  -

تاریخ انتشار 2015